Assay Method (T)

Assay Method of
 Tamsulosin Hydrochloride Tablet

Buffer	Take 1.25 gm Di-Sodium hydrogen orthophosphate & 0.125 gm Potassium di-hydrogen orthophosphate in 500ml of water & adjust the PH to 4.0 with dilute phosphoric acid.  Filter through 0.45 m membrane filter and Degasses.
Ratio	Methanol : Buffer = 60 : 40
Diluents	Methanol
Standard	Accurately weigh and transfer about 20 mg Tamsulosin HCl Working Standard in a 100-ml Volumetric flask. Add Diluents and shake for10 minutes and make the volume up to mark & mix well. Transfer 4 ml of this solution in a 100 ml volumetric flask and add diluents up to the  mark (100 ml) & mix well. Filter this solution with 0.45m membrane filters before injection
Sample	Weigh and powder 20 tablets. Take powder-containing equivalent to 0.4 mg of Tamsulosin HCl in a 50-ml volumetric flask. Add Diluents (Methanol HPLC grade) and shake for 30 minutes & degasses for 10 minutes and make the volume up to mark & mix well. Filter the solution  through whatman filter paper no.1 and half of   Filter this solution with 0.45m  membrane filters before injection
Condition	Waters Spherisorb 10.0 mm ODS2   4.6 ´ 250mm./ UV at 225 nm / 15 minutes.

Assay Method of
 Telmesartan INN Tablet

Buffer pH 3.5	Dissolve 3.4 gm Potassium Dihydrogen Phosphate in 1000 mL of water. Add 1.0 mL of Triethylamine and adjust pH to 3.5 with dilute ortho Phosphoric acid.
Ratio	Buffer 60 % : Acetonitryl 40 %
Diluents	Methanol
Standard	Weigh accurately about 50 mg Telmisartan WS and transfer it in a 100 ml volumetric flask. Add 50 ml of Methanol. Shake for 10 min. Then add sufficient Methanol to make 100 ml. Mix and filter this solution with 0.45mm membrane filter before injection.
Sample	Take 20 tablets, weigh and determine the Average weight. Then crush these tablets into fine powder using mortar and pastle. Take accurately weight of the Tablet powdered sample containing about 50 mg Telmisartan and transfer in a 100 ml volumetric flask. Add 60ml of Methanol. Shake for 20 min and add sufficient diluent to make 100 ml. Mix well and degassed for 10 minutes. Then filter the solution with whatman filter paper. Filter this resultant solution with 0.45 mm membrane filter before injection.
Condition	Luna C18 5µm. 4.6 × 250mm. / 230 nm / 1ml per minute / 20 mL / 25 minutes / 19 minutes (Approx.)

Assay Method of 
 Tenoxicam Tablet

Sample

Take powdered sample equivalent to 90 mg of Tenoxicam and dissolve in a mixture of 70 ml Glacial Acetic Acid and 5 ml of Formic Acid solution which is previously neutralized. Titrate with 0.1 N Perchloric acid and determine the end point potentiometrically using crystal violet as an indicator. 1 ml 0.1N Perchloric acid VS ≡ 33.74 mg of Tenoxicam

  Assay Method of

 Terbinafine Hydrochloride Tablet

Solvent	Methanol
Standard	50 mg ---® 100 ml ---® take 1 ml ---® 50 ml ---® take 5 ml ---® 50 ml
Sample	Same as Standard. Take absorbance at 223 nanometer.

Assay Method of
 Tetracycline Capsule

Sample

Weigh and powder 20 capsules. Take powder  equivalent to  50 mg of Tetracycline Hydrochloride  in 100 ml volumetric flask. Add 60 ml of 0.1N Hydrochloric acid  and shake for 20 minutes. Make the volume up to 100 ml with the same solvent . Mix and filter. Dilute 1 ml of the filtrate to 50 ml with  0.1N Hydrochloric acid . Measure absorbance at 274 nm , taking 0.1N Hydrochloric acid  as Blank . % of content  =  Absorbance  x 100 / conc. in g /100 ml x 387

Assay Method of
 Tiemoniun methylsulphate Tablet

Buffer	Add 1 ml triethylamine in 500 ml of water and adjust the PH  3.0( ±0.05) with dilute phosphoric acid.
Ratio	Acetonitrile : Buffer in the proportion of  70 : 30
Diluents	Acetonitrile : Buffer in the proportion of  70 : 30
Standard	Accurately  weigh and  transfer about 25 mg of Tiemoniun methylsulphate   working  standard  in a 100 ml volumetric flask .Add 70 ml diluents and shake for 10 minutes and make the volume up to the mark with the same solvent. Filter this solution with 0.20 m membrane filter before injection.
Sample	Weigh and make fine powder of  20 tablets. Take an accurately weighed portion of powder equivalent to 25 mg of Tiemoniun methylsulphate in a 100 ml volumetric flask. Add 70 ml of diluents and shake for 10 minutes . Adjust the volume to100 ml with the same solvent .Then degassed it for 15 minutes. Filter the solution by Whatman filter paper. Further  filter this solution with 0.20 m membrane filter before injection.
Condition	XTerra® RP18 ,5 mm    4.6 ´ 250mm./ UV at 235 nm / 8 minutes

Assay Method of
 Tiemoniun methylsulphate Injection

Solvent	water
Standard	50 mg ---® 100 ml ---® take 5 ml ---® 100 ml (Shake 20 minutes)
Sample	Same as Standard. Take absorbance at 235 nanometer.

                                                                 

Assay Method of
 Tizanidine Tablet

Buffer	Dissolve 6.8 gm Monobasic potassium phosphate[ KH 2  PO4 ]in 500 ml of water and make up to 1000 ml with  water. Adjust pH of this solution to 7.5 ± 0.1with potassium hydroxide.
Ratio	Accetonitrile : Buffer º 50 : 50
Diluents	Accetonitrile : Buffer º 20 : 80
Standard	Weight and transfer about 25 mg working standard of Tizanidine in a 100 ml  volumetric flask. Add 70 ml of solvent and shake for 20 minutes and dilute with solvent up to the mark and mix well. Take 5 ml solution in a 50 ml volumetric flask and dilute with solvent and mix well.Filter this solution with 0.45 m membrane filter before injection.
Sample	Take the weight of 20 tablets & determine the average weight. Crush the tablets into fine powder. Take an amount of powder sample equivalent to  25 mg Tizanidine in a 100 ml volumetric flask. Add 70 ml solvent and shake for 30 minutes and  dilute  with solvent up to the mark and mix well. Filter the solution by whatman filter paper and take 5 ml solution in a 50 ml volumetric flask and dilute with solvent and mix well. Finally filter this solution with 0.45 m  membrane filter before injection.
Condition	XTerra RP18 , 5 mm  4.6 ´ 250mm./ 40°C  / UV at 230 nm / 8 minutes.

Assay Method of 
 Theophylline Tablet & Syrup

Buffer	Transfer 1.36 gm of Sodium acetate trihydrate to a 1000 ml volumetric flask add about 200 ml of distilled water and shake until dissolution is complete and volume up to 1000 ml with distilled water .
Ratio	Mix buffer solution and acetonitrile  (70 : 30 ) filter through 0.45 m  membrane filter and degasses.
Diluents	Mobile Phase
Standard	Weight and transfer about 50 mg working standard of Theophylline  in a 50 ml volumetric flask. Add 30 ml of Mobile Phase and shake for 20 minutes and dilute with  Mobile Phase up to the mark and mix well. Take 5 ml solution in a 50 ml volumetric flask and up to the mark with Mobile Phase and mix well. Filter this  solution with 0.45 m membrane filter before injection.
Sample	Take the weight of 20 tablets & determine the average weight. Crush the tablets into fine powder. Take an amount of powder sample equivalent to  50 mg Theophylline in a 50 ml volumetric flask. Add 30 ml Mobile Phase and shake for 30 minutes and  dilute  with Mobile Phase up to the mark and mix well. Filter the solution by whatman filter paper and take 5 ml filtrate solution in a 50 ml volumetric flask and up to the mark with Mobile Phase and mix well. Finally filter this solution with 0.45 m  membrane filter before injection.
Condition	Waters Sphreisorb  ODS1 , 10 m  250 x 4.6 mm./ 40°C  / UV at 280 nm / Theophylline  = 3.5 minutes.

Assay Method of 
 Tolperiaone Hydrochloride Tablet

Sample

Dissolve powdered sample equivalent to 100 mg Tolperiaone Hydrochloride in previously neutralized mixture of 60 ml glacial acetic acid and acetic anhydride (40 ml + 20 ml) using crystal violet solution as indicator. Titrate with 0.1 M perchloric acid and determine the end point.Each ml of 0.1M perchloric acid VS is equivalent to 28.183 mg of Tolperiaone Hydrochloride.% of content =   V x F x 28.183 x 100 / W

Assay Method of 
 Tramadol Hydrochloride Suppository

Sample

Weigh 20 Suppositories and then cut in to small pieces. Take about 1.4 gm sample equivalent to 100 mg Tramadol Hydrochloride in a warm mixture of 25 ml Glacial Acetic Acid, 10 ml Acetic Anhydride and carryout non aqueous titration, determining the end point Potentiometrically. Perform a reagent blank use Crystal violet indicator if necessary. Each ml of 0.1 M Perchloric acid VS is equivalent to 29.98 mg of Tramadol Hydrochloride

Assay Method of
 Tramadol Hydrochloride Capsule & Injection

Solvent	Water
Standard	50 mg ----® 100 ml ----® take 10 ml ----® 50 ml  (Shake 15 minutes)
Sample	Same as standard. Take absorbance at 272 nm.

Assay Method of
 Trimetazidine Dihydrochloride Tablet

Buffer	Take 6.805  gm & Potassium Dihydrogen Phosphate in a 1000ml volumetric flask and adjust the volume with Distilled water & adjust the pH 4.5 with Phosphoric Acid.
Ratio	ACN : Buffer = 40: 60
Standard	Accurately weigh and transfer about 25 mg of working standard of Trimetazidine.Dihydrochloride  into 50 ml volumetric flask Add 35 ml of Methanol  & shake for 20 minutes and  dilute with Methanol up to the mark and then sonicate for 20  minutes Take 2 ml of the solution in a 50 ml volumetric flask. Volume up  to mark with Methanol .Filter this solution with 0.25 m membrane filter before injection.
Sample	Weigh of 10 tablets and make it  powder. Take powder-containing equivalent to 25 mg of Trimetazidine Dihydrochloride in a  50-ml volumetric flask. Add 35 ml of Methanol & shake for 20 minutes. Dilute with Methanol up to the mark then  Sonicate this Solution for 20   minutes & filter with Whitman filter paper .Take 2 ml of the solution in a 50 ml volumetric flask. Make the volume up  to mark with Methanol. Filter this solution with 0.25 mm membrane filter before injection.
Condition	Waters Spherisorb 10 mm ODS1  4.6 ´ 250mm./ 40º C / UV at 210 nm / 10 minutes.