Calcium Carbonate / Tablet
|
Sample
|
Take the amount of powder equivalent to about 40 mg of
calcium transfer to conical flask (250ml). Add 10ml of distilled water then
heat on water bath about 15-20 minute then cool it. Dissolve the residue by adding sufficient
(1-2 ml) 3N hydrochloric acid drop-wise, to achieve complete solution. Then
dilute with water to 150ml, add 15ml of 1N sodium hydroxide and 3-5 mg of
Hydroxy naphthol blue as indicator and titrate with 0.05M Edetatedisodium VS
until the solution is deep blue.1 ml 0.05M Edetate disodium º
2.004 mg of Calcium
% of content = V1 ´ F ´
2.004 ´ 100 / W
|
Calcium Lactate / Tablet
|
Sample
|
Dissolve a quantity of the powder containing 0.300 g of
Calcium Lactate Pentahydrate in 50 ml of water and titrate with 0.05 M
Disodium Edetate VS to within a few milliliters of the expected end point.
Add 8 ml of 5M Sodium Hydroxide and
0.05 g of Solochrome dark blue mixture and continue the titration until the
colour changes from pink to full blue.
Each ml of 0.05 M Disodium Edetate VS º
15.41 mg of Calcium lactate pentahydrate .
% of content =
Required volume of VS x F x 15.41x 100
/ Weight of Calcium Lactate Pentahydrate
|
Calcium Pantothenate /
Injection
|
Sample
|
Take
sample equivalent to 200 mg of Calcium Pantothenate in a 250 ml titration
flask. Add 200 ml water, 4 ml 10 M sodium hydroxide & 15 mg of calcon
carboxylic acid triturate. Titrate with 0.05 M Na2EDTA vs until the color
changes from violet to full blue. Each ml of 0.05 M Na2EDTA VS is equivalent
to 23.825 mg of Calcium Pantothenate .
Calculation:
V x F x 23.825 x 100 / weight of Calcium Pantothenate in mg.
|
Captopril / Tablet
|
Solvent
|
0.1M Sodium Hydroxide
|
|
Standard
|
50 mg ----® 100 ml ----®
take 2 ml ----®
50 ml (Shake 25 minutes)
|
|
Sample
|
Same as standard. Measure the absorbance at 238 nm
Use 0.1M Sodium Hydroxide as blank.
|
Carbamazepine / Tablet
|
Solvent
|
Ethanol
|
|
Standard
|
50 mg ----® 50 ml ----®
take 5 ml ----®
50 ml (Shake 10 minutes)
|
|
Sample
|
Same as standard. Measure the absorbance at 285 nm
Use 0.1M Sodium Hydroxide as blank.
|
Carvidilol / Tablet
|
Buffer
|
Dissolve 2.723 gm of Potassium dihydrogen
orthophosphate with 950 ml distilled
water into 1000 ml volumetric flask,
adjust the pH 2.0 ±
0.05 with Phosphoric acid & make
volume with distilled water up to 1000
ml.
|
|
Ratio
|
Buffer 65% : Acetonitrile 35%
|
|
Solvent
|
1st Dilution – Acetonitrile & 2nd Dilution – Acetonitrile 60% : Water 40%
|
|
Standard
|
25 mg ----® 50
ml ----®
take 2 ml ----®
25 ml (Shake 20 minutes)
|
|
Sample
|
Same as
standard.
|
|
Condition
|
C8 5.0 mm
4.6 x 250 mm / 55°C / UV at 240 nm / 8 minutes
|
Cefaclor / Powder for Suspension
|
Solvent
|
Water
|
|
Standard
|
100 mg ----® 100 ml ----®
take 2 ml ----®
100 ml (Shake 10 minutes)
|
|
Sample
|
Same as standard. Measure the absorbance at 265 nm
Use water as blank.
|
Cefepime HCl /
Injection
|
Buffer
|
Weigh
2.88 gm of Hexane –1 Sulphonic acid sodium salt.& transfer to a 1000 ml
volumetric flask. Add approx. 500 ml distilled water to dissolve completely &
volume up to the mark with distilled water &mix well. Adjust pH 4.0 with
dilute glacial acetic acid or Potassium Hydroxide (if required).
|
|
Ratio
|
Buffer ( 90% ) : Acetonitrile ( 10% )
|
|
Solvent
|
Mobile Phase
|
|
Standard
|
25 mg ----® 50
ml (Shake 20 minutes)
|
|
Sample
|
Same as
standard.
|
|
Condition
|
Waters
Spherisorb S10 C8, 4.6 x 250 mm / UV
at 254 nm / 15 minutes
|
Cefixime / Capsule + Powder
for Suspension
|
Solvent
|
Methanol
|
|
Standard
|
50 mg ----® 100 ml ----®
take 2 ml ----®
100 ml (Shake 20 minutes)
|
|
Sample
|
Same as standard. Measure the absorbance at 290 nm
Use methanol as blank.
|
Ceftazidim Pentahydrate / Injection
|
Buffer pH = 7.0
|
Dissolve
42.59 g anhydrous dibasic sodium phosphate and 27.22 g of monobasic potassium
phosphate in water to make 1000 ml of solution.
|
|
Ratio
|
Mix 70
ml of acetonitrile and 100 ml of pH 7 buffer and dilute with water to obtain 1000 ml of solution. Filter this
solution with 0.45 m membrane filter and degas.
|
|
Solvent
|
Mobile
Phase
|
|
Standard
|
Ceftazidime
Pentahydrate (with buffer) working standard equivalent to about 50 mg of
Ceftazidime 50 mg ----® 50 ml ----®
take 5 ml ----® 50 ml (Shake 20 minutes)
|
|
Sample
|
Same as
standard.
|
|
Condition
|
Waters
Spherisorb ODS1 10m,
4.6 x 250 mm / UV at 254 nm / 40m / 1.5 ml per
minutes / 8 minutes
|
Ceftriaxone /
Injection
|
Sample
|
Determine
the weight of the contents of 10 containers as described in the test for
uniformity of weight. Dissolve a quantity of the mixed contents of the 10
containers containing the equivalent of 0.05 g of ceftriaxone in sufficient
water to produce 100 ml. Dilute 2 ml to 100 ml with water.
Prepared
standard solution in the same way. Measure the absorbance of the resulting
solutions at 242 nm using water as blank . Concentration 0.001 %
|
Cefuroxime Axetil /
Tablet & Powder for Suspension
|
Solvent
|
Methanol
|
|
Standard
|
36 mg Cefuroxime Axetil equivalent 30 mg
Cefuroxime ----®
50 ml ----®
take 1 ml ----®
50 ml (Shake 15 minutes)
|
|
Sample
|
Same as standard. Measure the absorbance at 277 nm
Use methanol as blank.
|
Cephalexin /
Capsule
|
Sample
|
Weigh and powder 20 capsules. Take powder equivalent to 50 mg of Anhydrous Cephalexin in 100 ml
volumetric flask. Add 60 ml of water ,
and shake for 25 minutes. Make the volume up to 100 ml with water. Mix and
filter. Dilute 2 ml of the filtrate to 50 ml with water. Measure the absorbance of the
resulting solution at the maximum 262 nm,Calculate the content
of Cephalexin taking 232 as the value
of A ( 1%, cm ) at 262 nm.
% of content
= Absorbance of sample x
100 / Conc. in g / 100 ml
x 232
|
Cephradine / Injection
|
Sample
|
Determine the weight of the contents of 10 containers.
Dissolve a quantity of the mixed contents of the 10 containers containing the
equivalent of 0.1 g of cephradine in sufficient water to produce 100 ml.
Dilute 1 ml to 50 ml with 0.1 M hydrochloric acid. Prepare a standard
solution in the same concentration by using cephradine Reference Standard.
Measure the absorbances of the resulting solutions at the maximum at 257 nm
using 0.1 M hydrochloric acid as blank. Concentration 0.002 % w/v
|
Cephradine / Capsule & PFS
|
Solvent
|
Water
|
|
Standard
|
100 mg ----® 100 ml ----®
take 2 ml ----®
100 ml (Shake 15 minutes)
|
|
Sample
|
Same as standard. Measure the absorbance at 261 nm
Use water as blank.
|
Cetirizine Hydrochloride / Tablet
|
Solvent
|
0.1 M HCl
|
|
Standard
|
50 mg ----® 100 ml ----®
take 2 ml ----®
50 ml (Shake 20 minutes)
|
|
Sample
|
Same as standard. Measure the absorbance at 231 nm
Use 0.1 M HCl as blank.
|
Cetirizine Hydrochloride / Syrup
|
Mobile Phase
|
A = Methanol :
Water = 20 : 40 and
B = Acetonitrile
|
|
Solvent
|
Methanol : Water :
Acetonitrile = 20 :
40 : 40
|
|
Ratio
|
Binary gradient system
= A : B = 70 : 30
|
|
Standard
|
Dissolve 25 of Cetrizine Hydrochloride WS in 50 ml of
solvent . Dilute 5 ml to 25 ml with the same solvent.
|
|
Sample
|
Dilute 5 ml sample to 25 ml with the solvent.
|
|
Condition
|
Supilcosil Discovery
TM C 18 Column / 230 nm / 2.3 ml per minute / 40 µl
|
Cinchocaine HCl
|
Solvent
|
1M HCl
|
|
Standard
|
30 mg ----® 50 ml ----®
take 1 ml ----®
50 ml (Shake 15 minutes)
|
|
Sample
|
Same as standard. Measure the absorbance at 254 nm
Use 1M HCl as blank.
|
Cinnarizine / Tablet
|
Solvent
|
0.1M HCl
|
|
Standard
|
50 mg ----® 100 ml ----® take
1 ml ----®
50 ml (Shake 25 minutes)
|
|
Sample
|
Same as standard. Measure the absorbance at 254 nm
Use 0.1M HCl as blank.
|
Ciprofloxacin
/ PFS
|
Buffer
|
Add 1 ml triethylamine in 500
ml of water and adjust the PH
3.0( ±0.05) with dilute phosphoric acid.
|
|
Ratio
|
Acetonitrile : Buffer in the proportion of 35 : 65
|
|
Solvent
|
1st Dilution – 0.1M HCl & 2nd Dilution – Water
|
|
Standard
|
55.5 mg
Ciprofloxacin Hydrochloride
working standard equivalent to Ciprofloxacin 50 mg ----® 50
ml ----®
take 2.5 ml ----®
50 ml (Shake 10 minutes)
|
|
Sample
|
Same as standard.
|
|
Condition
|
Waters Spherissorb ODS1 10 mm
4.6 ´
250mm./ UV at 278 nm / 8 minutes
|
Ciprofloxacin
/ Tablet
|
Sample
|
Dissolve powdered sample equivalent to 100 mg
Ciprofloxacin in previously neutralized mixture of 60 ml glacial acetic acid
and 5% Mercury (II) Acetate (50 ml + 10 ml) using crystal violet solution as
indicator. Titrate with 0.1 M Perchloric acid and determine the end
point.Each ml of 0.1M perchloric acid VS is equivalent to 33.14 mg of
ciprofloxacin
% of content = V
x F x 33.14 x 100 / W
|
Ciprofloxacin
/ Tablet
|
Buffer
|
Add 1 ml triethylamine in 500
ml of water and adjust the PH
3.0( ±0.05) with dilute phosphoric acid.
|
|
Ratio
|
Acetonitrile : Buffer in the proportion of 35 : 65
|
|
Diluents
|
Acetonitrile : Buffer in the proportion of 35 : 65
|
|
Standard
|
Accurately weigh and transfer about 55.5 mg Ciprofloxacin Hydrochloride working
standard equivalent to 50 mg of
Ciprofloxacin in a 50 ml volumetric flask .Add diluents and shake for 25
minutes and make the volume up to the mark with the same solvent. Dilute 25
ml of this solution to 100ml with diluents. Filter this solution with 0.20 m
membrane filter before injection.
|
|
Sample
|
Take an accurately powder
equivalent to 250 mg of ciprofloxacin in 100 ml volumetric flask. Add 70 ml
of diluents and shake for 30 minutes & sonicate for 30 minutes.. Adjust
the volume to100 ml with diluents. Filter the solution by Whatman filter
paper. Take 10 ml of the filtrate in a 100 ml volumetric flask. Make the
volume up to the mark with diluents. Filter this solution with 0.20 m
membrane filter before injection.
|
|
Condition
|
Waters Spherissorb ODS1 10 mm
4.6 ´
250mm./ UV at 278 nm / 8 minutes
|
Chloramphenicol / Syrup
|
Sample
|
Dilute a weighed quantity containing the equivalent of
0.150 g of Chloramphenicol to 500 ml
with water , mix and allow to stand for 10 minutes . Mix again and
immediately dilute 5 ml of the oral suspension to 100 ml with ethanol (96 %). Measure the absorbance at the
maximum at 271 nm . Calculate the content of Chloramphenicol, taking 178 as
the value of A (1%, 1 cm) at the maximum at 271 nm .
|
|
Calculation
|
% of content
= Absorbance
x 100 / conc, in g per 100 ml x 178
|
Chlorhexidine Gluconate / Solution
|
M. Phase
|
Take accurately weighted 1.78 g
of Sodium Octane Sulphonate R in
a 1000ml volumetric flask add 240 ml
of Purified Water then add 105 ml of Glacial Acetic Acid shake for 5min. then
add 655 ml Methanol of HPLC Grade. Filter mobile phase with 0.45µm membrane
filter and degas for 30 minutes.
|
|
Standard
|
Take 1.25 ml Chlorhexidine Gluconate standard
solution (20% w/v) equivalent to 250 mg of Chlorhexidine Gluconate in
a 50 ml volumetric flask, make volume with the mobile phase. Take 2 ml of the
resulting solution and make 50 ml with
the same solvent. Calculate the concentration in
mg/ml. Filter this solution with 0.45 m membrane filter before
injection.
|
|
Sample
|
Take 2
ml sample containing 10 mg of Chlorhexidine Gluconate in a 50ml volumetric
flask, make volume with the mobile phase. Calculate the concentration in
mg/ml. Filter this solution with 0.45 m
membrane filter before injection.
|
|
Condition
|
Waters
Spherisorb 10 mm ODS1 4.6 ´
250mm./ 40º C / UV at 254 nm / 15 minutes.
|
Chloroquine Phosphate / Tablet
|
Standard
|
Weigh and powder 20 tablets . Dissolve a quantity of powder equivalent to 250 mg
of Chloroquine Phosphate in 10 ml of M
Sodium Hydroxide and extract with four 25 ml quantities of chloroform.
Combine the chloroform extracts and evaporate to a volume of about 10 ml. Add
40 ml of anhydrous acetic acid and carry out non aqueous titration,
determining the end point potetiometrically. Each ml of 0.1M perchloric acid
VS is equivalent to 25.79 mg of
Chloroquine Phosphate .
|
|
Calculation
|
% of Chloroquin Phosphate = No. of ml of 0.1M perchloric acid VS x F x
25.79 X 100 / 250 mg
|
Chloroquine Phosphate / Syrup
|
Solvent
|
Water
|
|
Standard
|
80 mg
----® 100 ml ----®
take 1 ml ----® 50 ml (Shake 15 minutes)
|
|
Sample
|
Same as
standard. Take absorbance at 343 nm.
|
Chlorpheniramine Maleate / Syrup
|
Sample
|
Take 10 ml of syrup equivalent to 4 mg of
Chlorpheniramine Maleate in a 250 ml separating funnel. Add 10 ml of M sodium
hydroxide . Extract two time each with 50 ml quantities of ether. Wash each
ether extract with the same 20 ml of water and extract with successive
quantities of 20 ,20 and 5 ml of 0.25 M sulphuric acid. Dilute the combined
acid extracts to 50 ml with 0.25 M sulphuric acid. Dilute 5 ml to 25 ml with
0.25 M sulphuric acid and measure the absorbance of the resulting solution at
the maximum at 265 nm .Calculate the content of chlorpheniramine maleate
taking 212 as the value of A (1% , 1 cm ) at the maximum at 265 nm .
% of
Chlorpheniramine maleate = Absorbance x 100 / conc. in gm per 100 ml x 212
|
Chlorpheniramine Maleate / Tablet
|
Sample
|
Weigh and powder 20 tablets. Dissolve a quantity of
powder equivalent to 25 mg of Chlorpheniramine
Maleate with 50ml volumetric flask. Add 30 ml of 0.05M
Sulphuric acid and shake for 15 minutes . Make volume up
to 50 ml with the same solvent. Mix & filter.Take 8 ml of the
filtrate equivalent to 4 mg of chlorpheniramine Maleate in a 250 ml separating funnel . Add
10ml of 1 M sodium hydroxide . Extract two time each with 50 ml quantities of
ether . Wash each ether extract with the same 20 ml of water and extract with
successive quantities of 20.20 and 5 ml of 0.25 M sulphuric acid . Dilute the
combined acid extracts to 50ml with 0.25 M sulphuric acid. Dilute 5ml to 25
ml with 0.25 M sulphuric acid and measure the absorbance of the resulting
solution at the maximum at 265 nm
Calculate content of chlorpheniramine maleate taking 212
as the value of A (1 % , 1 cm ) at the maximum at 265 nm.
% of chlorpheniramine maleate = Absorbance x
100 / conc. in gm per 100 ml x 212
|
Chlorpromazine Hydrochloride / Tablet & Injection
|
Standard
|
Carry out the following procedure protected from light.
To a quantity containing 50 mg of Chlorpromazine Hydrochloride add sufficient 0.1M hydrochloric acid to
produce 100 ml. Dilute 1 ml of the solution to 100 ml with 0.1M hydrochloric
acid and measure the absorbance of the
resulting solution at the maximum at 254 nm .
Calculate the content of
Chlorpromazine Hydrochloride
taking 915 as the value of A (1% , 1cm ) at the maximum at 254 nm
.
|
|
Calculation
|
% of
Chlorpromazine HCl =
Absorbance of Sample ´ 100 / Conc. Of spl. ´
915
|
Cisapride
/ Syrup
|
Standard
|
Prepare standard in the same concentration as sample,
taking cisapride RS.Concentration 0.001 %
|
|
Sample
|
Take suspension
equivalent to 25 mg of cisapride in a
250 ml separating funnel. Rinse the pipette with 5 ml of water. Extract two
times each with 40 ml of Chloroform. Wash the combined Chloroform extracts
with 25 ml of water. Collect the
extracts in a 400 ml beaker and evaporate to dryness on a water bath. Dissolve
the residue in 30 ml of Methanol and dilute to 50 ml with methanol. Mix .
Dilute 1 ml of the resulting solution to 50 ml with Methanol.Measure the
absorbance at 276 nm taking Methanol as reagent blank.
|
Clindamycin Phosphate
|
Buffer
|
Take 13.6 gm of KH2PO4 in a 1000 ml
volumetric flask containing 750 ml distilled water and dissolve by stirring.
Adjust the pH 4.0 by H3PO4.Finally make the volume 1000
ml by water.
|
|
Ratio
|
Buffer: Acetonitrile =78: 22
|
|
Diluent
|
Mobile Phase
|
|
Standard
|
356.07
mg of Clindamycin Phosphate º 300 mg of Clindamycin ----® 50 ml
----® take 5 ml ----® 50
ml (Shake 10 minutes)
|
|
Sample
|
Same as standard.
|
|
Condition
|
ODS1 10 m
4.6 x 250 mm / UV at 210 nm / 20 minutes
|
Clobazam / Tablet
|
Solvent
|
Methanol
|
|
Standard
|
20 mg
----® 100 ml ----®
take 2 ml ----® 100 ml (Shake 25 minutes)
|
|
Sample
|
Same as
standard. Take absorbance at nm.
|
Clonazepam /
Tablet & Solution
.
|
Sample
|
Take 1ml Pase Solution equivalent to 2.5 mg of Clonazepam in a 100 ml Amber
volumetric flask. And rinse the Pipette
with Isopropyl Alcohol. Add 70 ml
of
Isopropyl Alcohol and shake for 20
minutes. Make the volume up to the mark
(100 ml) with Isopropyl Alcohol &
mix well. (concentration = 0.0025 %).
Measure
the absorbance of sample solution in a UV at a wavelength of 310
nm using
blank solution.
|
|
Calculation
|
% of Clonazepam
= Absorbance of Sample ´ 100
/ Conc. Of spl. ´ 364
|
Clopidogrel +
Aspirin / Tablet
|
Buffer
|
Dissolve 2.0 gm
of Di-ammonium Hydrogen Orthophosphate
&1.0gm of Tetra-n Butyl Ammonium
Hydrogen Sulfate in
1000 ml distilled
water. Adjust pH 4.0±0.05 with Orthophosphoric acid.
|
|
Ratio
|
Accetonitrile : Buffer º 60 : 40
|
|
Diluents
|
Accetonitrile : Buffer º 60 : 40
|
|
Standard
|
48.94
mg of Clopidogrel Bisulfate º
37.5mg Clopidogrel +37.5 mg Aspirin ----® 50
ml (Shake 10 minutes)
|
|
Sample
|
Same as standard.
|
|
Condition
|
C18 5 mm 4.6
´
250mm./ UV at 210 nm / 15 minutes.Aspirin 3.20 min.(approx),
Clopidogrel 8 min.( approx).
|
Crotamiton + Permithrin / Lotion
For Crotamiton
|
Standard
|
Take 100 mg of Crotamiton working standard in a 100 ml
volumetric flask. Add 70 ml of cyclohexane. Shake for 15 minutes. Add
cyclohexane to make volume 100 ml. Take 1 ml of the solution in a 50 ml
volumetric flask. Make the volume up to mark (100 ml) with methanol. Measure
the absorbance at 242 nm taking cyclohexane as blank.
|
|
Sample
|
Take 1
ml of the lotion sample containing equivalent to 100 mg of
Crotamiton in a 100 ml volumetric flask. Add 70 ml of cyclohexane. Shake for
15 minutes. Add cyclohexane to make volume 100 ml. Filter the solution. Take
1 ml of filtered solution in a 50 ml volumetric flask. Make the volume up to
mark (50 ml) with cyclohexane. Measure the absorbance at 242 nm taking
cyclohexane as blank.
|
For Permithrin
|
Ratio
|
Methanol : Distilled Water = 80
: 20
|
|
Standard
|
Weigh and transfer about 100 mg working
standard of Permethrin in a 100 ml volumetric flask, dissolve in 75 ml of
Methanol then shake for 30 minutes.Adjust the volume 100 ml with the same
solvent. Filter this solution with 0.2 mm membrane filter before injection.
|
|
Sample
|
Take 2 ml
Lotion sample equivalent to 100 mg of Permethrin in a 100 ml volumetric
flask, dissolve in 75 ml of
Methanol
and shake for 30 minutes.Filter this solution with Whatman Filter paper and
finally filter with 0.2 mm
membrane
filter before injection.
|
|
Condition
|
Water Spherisorb , C-18 , 5m 4.6 x
250 mm / 400 C/ UV at 272 nm/15 minutes
|
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