Assay Method of
Econazole Nitrate Cream
Buffer
|
Prepare the phosphate buffer by
dissolving 2.5 g of potassium dihydrogen orthophosphate and 2.5 g of
dipotassium hydrogen orthophosphate in 1000 ml of water.
|
Ratio
|
Phosphate buffer: Methanol = 1
: 3
|
Standard
|
Weigh and
transfer about 100 mg working standard of Econazole Nitrate in a 100 ml
volumetric flask, dissolve in 75 ml of Methanol then shake for 15 minutes and
dilute with phosphate buffer up to the mark ( 100 ml) and mix well. Transfer
10 ml of this solution into a 100 ml volumetric flask and up to mark with
mobile phase.Filter this solution with 0.2 mm membrane filter before injection.
|
Sample
|
Take an amount of cream sample equivalent 10 mg of
Econazole Nitrate in a 100 ml volumetric flask, dissolve in
75 ml of Methanol and warm on a water-bath for 30
seconds, shake for 1 minute, repeat the process twice and add
25 ml phosphate buffer. Cool in a ice bath for 15
minutes. Filter this solution with Whatman Filter paper then
centrifuge for 10 minutes and use the supernant
liquid. Filter this solution with 0.2 mm membrane filter before
injection.
|
Condition
|
ODS2 , 10 m 4.6 x 250 mm / 400
C/ UV at 232 nm/15 minutes
|
Assay Method of
Entecavir Monohydrade Tablet
Buffer
|
Take
1000 ml of Distilled water & adjust the PH to 3.5 with Glacial Acetic acid. Filter through
0.45 m membrane filter and Degasses.
|
Ratio
|
Acetonitrile: Buffer = 50 : 50
|
Diluents
|
Buffer Solution
|
Standard
|
Accurately weigh and transfer Entecavir
Monohydrade equivalent to 20 mg of Entecavir Working Standard in a 100-
mlVolumetric flask. Add Diluents (Buffer) and
shake for 30 minutes and make the volume up to mark & mix well.
Transfer 10 ml of this solution in a 100 ml
volumetric flask and add diluents up
to the mark (100 ml) & mix well. Filter
this
solution with 0.25m membrane filters before
injection
|
Sample
|
Weigh
and powder 20 tablets. Take powder-containing equivalent to 0.5 mg of
Entecavir in a 25-ml volumetric flask. Add Diluents (Buffer) and shake for 30
minutes & Sonicate for 10 minutes and make the volume up to mark &
mix well. Filter the solution through whatman filter paper no.1.and then
filter this solution with 0.25m membrane filters before injection
|
Condition
|
Waters
Spherisorb 5 mm ODS2 4.6 ´
250mm./ UV at 224 nm / 15 minutes.
|
Assay Method of
Escitalopram Tablet
Standard
|
Dissolve about 32 mg of Escitalopram Oxalate WS equivalent
to 25 mg of Escitalopram in 50 ml volumetric
flask then add 30 ml methanol & shake for 15 minutes then take 1 ml
solution in 50 ml flask and up to mark with same solvent.
|
Sample
|
Take sample powder equivalent to 25 mg of Escitalopram in 50 ml volumetric flask then
add 30 ml methanol & shake for 15 minutes then filter by whattman filter
paper and take 1 ml solution in 50 ml flask and up to mark with same solvent.
Measure the absorbance of sample and standard solution at 238 nm using
methanol as blank.
|
Assay Method of
Esomeprazole Tablet
Standard
|
Dissolve about 25 mg of Esomeprazole WS in 60 ml of
methanol in a amber colored 100 ml volumetric flask and dilute to 100 ml with
same solvent. Dilute 2 ml to 50 ml with methanol.
|
Sample
|
Shake a quantity of powder containing 25 mg of
Esomeprazole with 60 ml of methanol in a amber colored 100 ml volumetric
flask for 20 minutes, add sufficient methanol to produce 100 ml and filter.
Dilute 2 ml of the filtrate to 50 ml with methanol. Measure the absorbance of
sample and standard solution at 302 nm using methanol as blank.
|
Assay Method of
Eszopiclone Tablet
Buffer
|
Dissolve 1.76 gm of Di Sodium hydrogen
orthophosphate & 13.61 gm Potassium dihydrogen orthophosphate with 950
ml
distilled water into 1000 ml volumetric flask, adjust the pH 3.5 ±
0.05 with dilute Phosphoric acid &
make volume
with distilled water up to
1000 ml.
|
Ratio
|
Acetonitrile: Buffer = 40 : 60
|
Diluents
|
Buffer Solution
|
Standard
|
Accurately weigh and transfer about 10 mg of
working standard of Eszopiclone into 100 ml volumetric flask. Add 75
ml of mobile phase
& shake for 40 minutes and dilute with mobile phase up to the mark
and mix well. Filter this
solution
with 0.45 m membrane filter before injection.
|
Sample
|
Weigh and
powder 20 Tablets. Take powder containing equivalent to 10 mg of Eszopiclone
into 100 ml volumetric flask. Add 75 ml of mobile phase & shake for 40
minutes and dilute with mobile phase up to the mark and mix well. Filter the
solution by whatman filter paper & finally filter this solution with 0.45
m
membrane filter before injection.
|
Condition
|
Waters Spherisorb 5 mm
ODS2 4.6 ´
250mm./ UV at 305 nm / 10 minutes.
Eszopiclone
: 4.0 minutes (approximately)
|
Assay Method of
Etodolac Tablet
Standard
|
Accurately weigh and transfer about 35 mg of working
standard of Etodolac into 50 ml
Volumetric flask Add 35 ml of Ethanol
& shake for 15 minutes and
dilute with Ethanol up to the mark and then Sonicate this solution for 15 minutes Take 2 ml of the solution in a 50
ml volumetric flask. Make the volume up
to mark ( 50 ml ) with Ethanol. (Concentration: 0.028 mg/ml). λ max
= 280 nm
|
Sample
|
Weigh of 10 tablets and make it powder. Take
powder-containing equivalent to 35 mg of Etodolac in a
50-ml volumetric flask. Add 35 ml of Ethanol & shake for 15
minutes. Dilute with Ethanol up to the mark then Sonicate this Solution for 15 minutes & filter with Whitman filter
paper .Take 2 ml of the solution in a 50 ml volumetric flask. Make the volume up to mark ( 50 ml ) with Ethanol.
(Concentration: 0.028 mg/ml).λ max = 280 nm
|
Assay Method of
Etoricoxib Tablet
Standard
|
Weigh
accurately and transfer about 20 mg Etoricoxib of working standard in a
100-ml volumetric flask. Add 70 ml of methanol and shake for 10 minutes and
then make the volume up to mark with methanol. Then dilute 2 ml of this
solution to 50 ml with Methanol. Measure the absorbance of the standard at λ max
235 nm taking methanol as a blank.
|
Sample
|
Weigh
and powder the 20 tablets. Take powder equivalent to 20 mg of Etoricoxib in a
100-ml volumetric flask. Add 70 ml of
methanol and shake for 20 minutes and perform sonication for 20 minutes and
then make the volume up to mark with methanol. Filter this solution with
Whatman filter paper. Dilute 2 ml of this solution to 50 ml with Methanol
Measure the absorbance of the standard at λ max 235 nm taking
methanol as a blank.
|
No comments:
Post a Comment