Monday, June 17, 2013

Sterilization, HVAC, Autoclave & DHS



1.What is sterilization? Mention the duration sterility test.
Sterilization is a process or technique to remove bacteria and all types of viable microorganism.
Duration: 14 days

2. Write the types of Sterilization.
Type of Sterilization:
Terminal Sterilization  
1) Steam Heat sterilization 
2) Dry Heat sterilization 
Others sterilization
3) Gas sterilization 
4) Sterilization by ionizing Radiation 
5) Sterilizing by Filtration 
6) Aseptic Processing

3.Write the functions & use of HVAC system.

Functions and use of HVAC


Heating, Ventilating and Air conditioning system is used for temperature and humidity control with in a manufacturing environment. It includes air handling units, air distribution network, air-cooling and heating system, air filtration, equipment control system, monitoring and alarm decreases

4.Write the conditions of sterilization by Autoclave & DHS.

Condition of sterilization by Autoclave & DHS


121 0 C 15 lbs pressure per square inch 15 minutes
200 o C for 1 Hour.

21. What are the points to be checked during validation of DHS/ Autoclave?
Points to be checked during validation of DHS/Autoclave

Checked by biological indicator (Bacillus sublitis) and endotoxin indicator for DHS
Checked by biological indicator (Bacillus stearothermophilus).

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